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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
04/03/2020 |
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Actualizado : |
04/03/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
OLIVEIRA, L.G.S.; BOABAID, F.M.; KISIELIUS, V.; RASMUSSEN, L.H.; BURONI, F.; LUCAS, M.; SCHILD, C.; LÓPEZ, F.; MACHADO, M.; RIET-CORREA, F. |
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Afiliación : |
LUIZ GUSTAVO SCHNEIDER DE OLIVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; FABIANA MARQUES BOABAID, Polo de Desarrollo Universitario Del Instituto Superior de La Carne, Centro Universitario Regional (CENUR) Noreste, Universidad de La República, Tacuarembó, Uruguay; VAIDOTAS KISIELIUS, Department of Technology, University College Copenhagen, Copenhagen, Denmark; LARS HOLM RASMUSSEN, Department of Technology, University College Copenhagen, Copenhagen, Denmark; FLORENCIA BURONI ZENI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; División de Laboratorios Veterinarios "Miguel C. Rubino" Regional Norte, Ministerio de Ganadería Agricultura y Pesca (MGAP), Tacuarembó, Uruguay; MARTÍN LUCAS FONSECA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIANA LÓPEZ, División de Laboratorios Veterinarios "Miguel C. Rubino" Regional Norte, Ministerio de Ganadería Agricultura y Pesca (MGAP), Tacuarembó, Uruguay; MIZAEL MACHADO DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Hemorrhagic diathesis in cattle due to consumption of Adiantopsis chlorophylla (Swartz) Fée (Pteridaceae). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Toxicon: X, March 2020, Volume 5, Article number 100024. OPEN ACCESS. Doi: https://doi.org/10.1016/j.toxcx.2020.100024 |
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ISSN : |
2590-1710 |
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DOI : |
10.1016/j.toxcx.2020.100024 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 November 2019 / Revised 12 January 2020 / Accepted 13 January 2020 / Available online 23 January 2020.
Corresponding author: Franklin Riet-Correa; email: franklin.riet@pq.cnpq.br
The authors acknowledge the financial support of the National Institute of Agricultural Research (INIA) of Uruguay (Project CL 44), as well as the Horizon 2020 research and innovation programme under the Marie Sk?odowska-Curie grant agreement No. 722493 ( www.natoxaq.eu ). Furthermore, we would like to acknowledge to Eduardo Marchesi, from the Agronomy Faculty of the Republic University, for the identification of the plant, and the assistance of laboratory technicians Jimena Bertorello Martinez (University College Copenhagen), Manuel Taño and Alejandra Custodio (INIA), Blanca Diaz and Luis Moreno (MGAP). |
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Contenido : |
ABSTRACT.
An outbreak of acute febrile syndrome associated with coagulopathy and severe pancytopenia occurred in cattle grazing in paddocks with high infestation by Adiantopsis chlorophylla. The administration of the plant to a calf reproduced the same signs and lesions seen in spontaneous cases. Similar syndromes are caused by ptaquiloside from bracken fern. Traces of the ptaquiloside-like molecule caudatoside were detected together with 0.03?0.24 mg/g of it's degradation product pterosin A, in dry fronds of the plant. In conclusion, A. chlorophylla is a cause of hemorrhagic diathesis in cattle.
© 2020 The Authors |
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Palabras claves : |
Cattle diseases; Caudatoside; Fern toxicity; Hematopoietic diseases; PLATAFORMA DE SALUD ANIMAL. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
https://www.sciencedirect.com/science/article/pii/S2590171020300023/pdfft?md5=349f77e276f03990a9f216827a370f63&pid=1-s2.0-S2590171020300023-main.pdf
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Marc : |
LEADER 02442naa a2200325 a 4500
001 1060891
005 2020-03-04
008 2020 bl uuuu u00u1 u #d
022 $a2590-1710
024 7 $a10.1016/j.toxcx.2020.100024$2DOI
100 1 $aOLIVEIRA, L.G.S.
245 $aHemorrhagic diathesis in cattle due to consumption of Adiantopsis chlorophylla (Swartz) Fée (Pteridaceae).$h[electronic resource]
260 $c2020
500 $aArticle history: Received 30 November 2019 / Revised 12 January 2020 / Accepted 13 January 2020 / Available online 23 January 2020. Corresponding author: Franklin Riet-Correa; email: franklin.riet@pq.cnpq.br The authors acknowledge the financial support of the National Institute of Agricultural Research (INIA) of Uruguay (Project CL 44), as well as the Horizon 2020 research and innovation programme under the Marie Sk?odowska-Curie grant agreement No. 722493 ( www.natoxaq.eu ). Furthermore, we would like to acknowledge to Eduardo Marchesi, from the Agronomy Faculty of the Republic University, for the identification of the plant, and the assistance of laboratory technicians Jimena Bertorello Martinez (University College Copenhagen), Manuel Taño and Alejandra Custodio (INIA), Blanca Diaz and Luis Moreno (MGAP).
520 $aABSTRACT. An outbreak of acute febrile syndrome associated with coagulopathy and severe pancytopenia occurred in cattle grazing in paddocks with high infestation by Adiantopsis chlorophylla. The administration of the plant to a calf reproduced the same signs and lesions seen in spontaneous cases. Similar syndromes are caused by ptaquiloside from bracken fern. Traces of the ptaquiloside-like molecule caudatoside were detected together with 0.03?0.24 mg/g of it's degradation product pterosin A, in dry fronds of the plant. In conclusion, A. chlorophylla is a cause of hemorrhagic diathesis in cattle. © 2020 The Authors
653 $aCattle diseases
653 $aCaudatoside
653 $aFern toxicity
653 $aHematopoietic diseases
653 $aPLATAFORMA DE SALUD ANIMAL
700 1 $aBOABAID, F.M.
700 1 $aKISIELIUS, V.
700 1 $aRASMUSSEN, L.H.
700 1 $aBURONI, F.
700 1 $aLUCAS, M.
700 1 $aSCHILD, C.
700 1 $aLÓPEZ, F.
700 1 $aMACHADO, M.
700 1 $aRIET-CORREA, F.
773 $tToxicon: X, March 2020, Volume 5, Article number 100024. OPEN ACCESS. Doi: https://doi.org/10.1016/j.toxcx.2020.100024
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Registro original : |
INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
12/07/2022 |
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Actualizado : |
12/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
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Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
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Complemento del título : |
Chapter 16. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
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ISBN : |
Online: 978-1-0716-2301-5 |
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DOI : |
10.1007/978-1-0716-2301-5_16 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
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Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02176naa a2200349 a 4500
001 1063414
005 2022-07-12
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-1-0716-2301-5_16$2DOI
100 1 $aKALDS, P.
245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource]
260 $c2022
520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock.
650 $aCABRAS
650 $aOVEJA
650 $aPRODUCCION DE CARNE
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aGoats
653 $aKnockout
653 $aMeat production
653 $aMicroinjection
653 $aMSTN
653 $aSheep
653 $aSmall ruminants
700 1 $aCRISPO, M.
700 1 $aTESSON, L.
700 1 $aANEGÓN, I.
700 1 $aCHEN KEY, Y.
700 1 $aWANG, X.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
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